Inhibitory Effect of Eicosapentaenoic Acid on the Migration of the Esophageal Squamous Cell Carcinoma Cell Line TE-1.

Abstract

BACKGROUND/AIM

eicosapentaenoic acid (EPA) inhibits NF-ĸB activation and IL-6 production in TE-1 esophageal cancer cells. NF-ĸB is related to cancer cell migration. The aim of this study is to evaluate whether epa has a metastasis suppressing effect. Herein, we investigated EPA-treated TE-1 cell migration using TAXIScan.

MATERIALS AND METHODS

EZ-TAXIScan® was used to verify whether epa inhibits cancer cell chemotaxis.

RESULTS

Using 50% fetal bovine serum (chemoattractant) without epa (positive control), average velocity was 0.306±0.084 μm/min compared to 0.162±0.067 μm/min without chemoattraction (negative control). Directionalities of positive and negative controls were 1.039±0.152 and 0.488±0.251 radians, respectively, indicating a significant increase in migration of the positive control compared to that of the negative control. Average velocities were 0.306±0.084 (no EPA), 0.288±0.078 (100 μM EPA), and 0.240±0.054 200 μM (EPA) μm/min, indicating that epa reduced velocity dose-dependently. Average directionalities were 1.039±0.152 (no EPA), 0.967±0.164 (100 μM EPA), and 0.901±0.146 (200 μM EPA) radians, indicating that epa also inhibited directionality dose-dependently.

CONCLUSION

epa suppresses directional migration of TE-1 cells.